ICSI significantly improved the pregnancy rate of patients with a high sperm DNA fragmentation index
نویسندگان
چکیده
OBJECTIVE Correlations between semen parameters and sperm DNA fragmentation index (DFI) were investigated to identify characteristics of sperm without DNA damage that could be used in selecting sperm for intracytoplasmic sperm injection (ICSI). Pregnancy outcomes were compared to determine whether in vitro fertilization (IVF) or ICSI is a better choice for patients who have sperm with a high-DFI. METHODS Semen analysis was carried out in 388 patients who visited our IVF center for the first time to investigate correlations between sperm DFI and semen parameters. In addition, 1,102 IVF cycles in 867 patients were carried out in the present study; 921 cycles in the low-DFI group (DFI <30%) and 181 cycles in the high-DFI group (DFI ≥30%). Both the low- and high-DFI groups were subdivided into IVF and ICSI cycle groups. RESULTS Sperm DFI showed significant inverse correlations with sperm motility (r=-0.435, p<0.001) and morphology (r=-0.153, p<0.05). Sperm DFI also showed significant correlations with rapid motility (r=-0.436, p<0.001), and the kinetic parameters of average-path velocity (r=-0.403) and linearity (r=-0.412). Although there was no significant difference in the pregnancy rates between IVF (48.6%) and ICSI (44.8%) in the low-DFI group, the pregnancy rate of ICSI cycles (44.8%, p<0.05) was significantly higher than IVF cycles (25.0%) in the high-DFI group. No significant difference was observed in the abortion rates between the low-DFI (52 of 921, 5.6%) and high-DFI groups (7 of 181, 3.8%). CONCLUSION ICSI is a better choice than IVF for improving the pregnancy outcomes of patients who have sperm with a high DFI.
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Effects of Sperm DNA Fragmentation on Semen Parameters and ICSI Outcome Determined by an Improved SCD Test, Halosperm
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